(Journal Article): The cyclosporine A-induced decrease in rat renal calbindin-D28kDa protein as a consequence of a decrease in its mRNA.
 
Grenet O, Varela MC, Staedtler F, Steiner S (Preclinical Safety, Novartis Pharma AG, Basel, Switzerland.)
 
IN: Biochem Pharmacol 1998; 55(7):1131-1133
Impact Factor(s) of Biochem Pharmacol: 3.436 (2004), 2.993 (2003), 3.542 (2002), 3.34 (2001)

Fulltext:    HTML  PDF

ABSTRACT: Cyclosporine A (CsA) is a potent immunosuppressant with the drawback of renal side-effects. We recently reported that relatively high doses of CsA markedly decreased the calcium-binding protein calbindin-D28kDa in kidneys of male Wistar rats, and showed that this decrease could be associated with some of the drug-induced adverse renal effects. To investigate the events leading to this decrease, the calbindin-D28kDa mRNA level in kidneys of rats treated with 15 or 50 mg/kg/day CsA for 12 days was analysed by reverse transcription followed by polymerase chain reaction. At both doses, a marked dose-dependent decrease in the calbindin-D28kDa mRNA level was found, one very similar to the decrease measured in the calbindin-D28kDa protein abundance. Thus, the CsA-mediated down-regulation of the renal calbindin-D28kDa protein is most likely the result of a decrease in the calbindin-D28kDa mRNA level.

TYPE OF PUBLICATION: Original article

Articles citing this article:

• Thongboonkerd V, Zheng S, McLeish KR, Epstein PN, Klein JB. Proteomic Identification and Immunolocalization of Increased Renal Calbindin-D28k Expression in OVE26 Diabetic Mice. Rev Diabetic Stud 2005. 2: 19-26.
  » Diabetes OD » Diabetic Complications » Renal » Hypercalciuria » Compensatory Mechanisms » Calcium Homeostasis » Journal Article