(Journal Article): Regeneration of pancreatic beta cells from intra-islet precursor cells in an experimental model of diabetes.
 
Guz Y, Nasir I, Teitelman G (Department of Anatomy and Cell Biology, State University of New York, Health Science Center at Brooklyn, Brooklyn, New York 11203, USA.)
 
IN: Endocrinology 2001; 142(11):4956-4968
Impact Factor(s) of Endocrinology: 5.151 (2004), 5.063 (2003), 5.095 (2002), 4.971 (2001)

Fulltext:    HTML  PDF

ABSTRACT: We previously reported that new beta cells differentiated in pancreatic islets of mice in which diabetes was produced by injection of a high dose of the beta cell toxin streptozotocin (SZ), which produces hyperglycemia due to rapid and massive beta cell death. After SZ-mediated elimination of existing beta cells, a population of insulin containing cells reappeared in islets. However, the number of new beta cells was small, and the animals remained severely hyperglycemic. In the present study, we tested whether restoration of normoglycemia by exogenous administered insulin would enhance beta cell differentiation and maturation. We found that beta cell regeneration improved in SZ-treated mice animals that rapidly attained normoglycemia following insulin administration because the number of beta cells per islet reached near 40% of control values during the first week after restoration of normoglycemia. Two presumptive precursor cell types appeared in regenerating islets. One expressed the glucose transporter-2 (Glut-2), and the other cell type coexpressed insulin and somatostatin. These cells probably generated the monospecific cells containing insulin that repopulated the islets. We conclude that beta cell neogenesis occurred in adult islets and that the outcome of this process was regulated by the insulin-mediated normalization of circulating blood glucose levels.

TYPE OF PUBLICATION: Original article

Articles citing this article:

• Hao E, Tyrberg B, Itkin-Ansari P, Lakey JR, Geron I, Monosov EZ, Barcova M, Mercola M, Levine F. Beta-cell differentiation from nonendocrine epithelial cells of the adult human pancreas. Nat Med 2006. 12: 310-316.
  » Diabetes OD » Regeneration of Islets » Stem Cells » Other Cell Sources » Progenitors » Pancreatic Epithelial Cells » Journal Article