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(Journal Article): Selective elimination of fibroblasts from cultures of normal human melanocytes.
Halaban R, Alfano FD
IN:
In Vitro
1984; 20(5):447-450
ABSTRACT: The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin ( G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 micrograms/ml for two days results in pure cultures of normal human melanocytes.
TYPE OF PUBLICATION: Original article
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